Mouse IgG F(ab)2 Isotype Control [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97223
![SDS-PAGE: Mouse IgG F(ab)2 Isotype Control [HRP] [NBP1-97223]](https://resources.bio-techne.com/images/products/Mouse-IgG-F-ab-Isotype-Control-HRP-SDS-Page-NBP1-97223-img0001.jpg "SDS-PAGE: Mouse IgG F(ab)2 Isotype Control [HRP] [NBP1-97223]")
Key Product Details
Species Reactivity
Mouse
Applications
SDS-Page
Label
HRP
Antibody Source
Mouse IgG
Concentration
LYOPH mg/ml
Product Summary for Mouse IgG F(ab)2 Isotype Control [HRP]
Specificity
No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
Host
Mouse
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Mouse IgG, anti-Mouse IgG F(ab')2 and anti-Mouse Serum
Scientific Data Images for Mouse IgG F(ab)2 Isotype Control [HRP]
SDS-PAGE: Mouse IgG F(ab)2 Isotype Control [HRP] [NBP1-97223]
SDS-Page: Mouse IgG F(ab) Isotype Control [HRP] [NBP1-97223] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab')2 Fragment Peroxidase Conjugated. Lane 5: Mouse IgM Kappa Myeloma Protein. Load: 1 ug per lane.Applications for Mouse IgG F(ab)2 Isotype Control [HRP]
Application
Recommended Usage
SDS-Page
1:100 - 1:2000
Application Notes
This product can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab)2
Additional IgG F(ab)2 Products
Product Documents for Mouse IgG F(ab)2 Isotype Control [HRP]
Product Specific Notices for Mouse IgG F(ab)2 Isotype Control [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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