Chicken IgG F(ab) Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96773
![SDS-PAGE: Chicken IgG F(ab) Isotype Control [NBP1-96773]](https://resources.bio-techne.com/images/products/Chicken-IgG-F-ab-Isotype-Control-SDS-Page-NBP1-96773-img0002.jpg "SDS-PAGE: Chicken IgG F(ab) Isotype Control [NBP1-96773]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Chicken
Applications
ELISA, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Chicken IgG
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
No reaction was observed against anti-Chicken IgG F(c) or anti- Papain.
Host
Chicken
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken IgG, anti-Chicken IgG F(ab')2 and anti-Chicken Serum
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Scientific Data Images for Chicken IgG F(ab) Isotype Control
SDS-PAGE: Chicken IgG F(ab) Isotype Control [NBP1-96773]
SDS-Page: Chicken IgG F(ab) Isotype Control [NBP1-96773] - SDS-PAGE of Chicken IgG Fab Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: IgG at 72 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 75 kDa. Observed F(c) Fragment migrates slightly higher. Other bands: Chicken IgG heavy chain alternative splicing variant at approximately 40 kDa in Lane 1.Applications for Chicken IgG F(ab) Isotype Control
Application
Recommended Usage
ELISA
1:100 - 1:2000
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
No Preservative
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: IgG F(ab)
Additional IgG F(ab) Products
Product Documents for Chicken IgG F(ab) Isotype Control
Product Specific Notices for Chicken IgG F(ab) Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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