Chicken IgG F(ab) Isotype Control [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97164
Forumulation
Catalog #
Key Product Details
Species Reactivity
Chicken
Applications
SDS-Page
Label
Biotin
Antibody Source
Chicken IgG
Concentration
LYOPH mg/ml
Product Specifications
Specificity
No reaction was observed against anti-Chicken IgG F(c) or anti-Papain.
Host
Chicken
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken IgG, anti-Chicken IgG F(ab')2 and anti-Chicken Serum
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Chicken IgG, anti-Chicken IgG F(ab')2 and anti-Chicken Serum
Scientific Data Images for Chicken IgG F(ab) Isotype Control [Biotin]
SDS-PAGE: Chicken IgG F(ab) Isotype Control [Biotin] [NBP1-97164]
SDS-Page: Chicken IgG F(ab) Isotype Control [Biotin] [NBP1-97164] - Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment. Lane 3: Reduced Chicken IgG Fab Fragment Biotin Conjugated. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane.Applications for Chicken IgG F(ab) Isotype Control [Biotin]
Application
Recommended Usage
SDS-Page
1:100 - 1:2000
Application Notes
This product has been tested by SDS-PAGE and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG F(ab)
Additional IgG F(ab) Products
Product Documents for Chicken IgG F(ab) Isotype Control [Biotin]
Product Specific Notices for Chicken IgG F(ab) Isotype Control [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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