Canine IgG Fc Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96769
Conjugate
Catalog #
Key Product Details
Species Reactivity
Canine
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Canine IgG
Format
Purified
Concentration
Please contact technical services for concentration.
Product Specifications
Specificity
No reaction was observed against anti-Dog IgG F(ab')2 or anti-Papain.
Host
Canine
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum, anti-Dog IgG and anti-Dog IgG F(c)
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Applications for Canine IgG Fc Isotype Control
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
Please contact technical services for concentration.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Canine IgG Fc Isotype Control
Product Specific Notices for Canine IgG Fc Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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