Guinea Pig IgG Fc Isotype Control [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97273
Forumulation
Catalog #
Key Product Details
Species Reactivity
Guinea Pig
Applications
Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, SDS-Page
Label
Biotin
Antibody Source
Guinea Pig IgG
Concentration
LYOPH mg/ml
Product Specifications
Specificity
No reaction was observed against anti-Guinea Pig IgG F(ab')2 or anti-Papain.
Host
Guinea Pig
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Guinea Pig IgG, anti-Guinea Pig IgG F(c) and anti-Guinea Pig Serum
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Guinea Pig IgG, anti-Guinea Pig IgG F(c) and anti-Guinea Pig Serum
Scientific Data Images for Guinea Pig IgG Fc Isotype Control [Biotin]
SDS-PAGE: Guinea Pig IgG Fc Isotype Control [Biotin] [NBP1-97273]
SDS-Page: Guinea Pig IgG Fc Isotype Control [Biotin] [NBP1-97273] - SDS PAGE Results of Guinea Pig IgG Fc isotype control [Biotin]. Guinea Pig IgG Fc isotype control [Biotin] (1) 100ng, (2) 33.33ng, (3), 11.11ng, (4) 3.70, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30 mins at RT. Blocking buffer at RT for 90 mins. Exposure: 5 secs.Applications for Guinea Pig IgG Fc Isotype Control [Biotin]
Application
Recommended Usage
Fluorophore-linked immunosorbent assay
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
SDS-Page
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This product has been tested in SDS-Page and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Guinea Pig IgG Fc Isotype Control [Biotin]
Product Specific Notices for Guinea Pig IgG Fc Isotype Control [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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