Chicken IgG Fc Isotype Control [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97284
Forumulation
Catalog #
Key Product Details
Species Reactivity
Chicken
Applications
SDS-Page
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Chicken IgG
Concentration
LYOPH mg/ml
Product Summary for Chicken IgG Fc Isotype Control [FITC]
Specificity
No reaction was observed against anti-Chicken IgG F(ab')2 or anti-Papain.
Host
Chicken
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken IgG, anti-Chicken IgG F(c) and anti-Chicken Serum
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken IgG, anti-Chicken IgG F(c) and anti-Chicken Serum
Scientific Data Images for Chicken IgG Fc Isotype Control [FITC]
SDS-PAGE: Chicken IgG Fc Isotype Control [FITC] [NBP1-97284]
SDS-Page: Chicken IgG Fc Isotype Control [Fluorescein] [NBP1-97284] - Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Chicken IgG Whole Molecule. Lane 2: Reduced Chicken IgG F(c) Fragment Fluorescein Conjugated. Lane 3: Reduced Chicken IgG Fab Fragment. Lane 4: Reduced Chicken IgM Whole Molecule. Load: 1 ug per lane.Applications for Chicken IgG Fc Isotype Control [FITC]
Application
Recommended Usage
SDS-Page
1:100 - 1:2000
Application Notes
This product has been tested by SDS-PAGE and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG Fc
Additional IgG Fc Products
Product Documents for Chicken IgG Fc Isotype Control [FITC]
Product Specific Notices for Chicken IgG Fc Isotype Control [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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