Key Product Details

Species Reactivity

Goat

Applications

Block/Neutralize, Control, ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Janelia Fluor 549

Antibody Source

Polyclonal Goat IgG

Concentration

5.0 mg/ml

View all IgG Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Goat IgG Isotype Control [Janelia Fluor 549]

Application
Recommended Usage

ELISA

1:100 - 1:400

Flow Cytometry

1:100 - 2000

Fluorophore-linked immunosorbent assay

1:100 - 12000

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50 mM Sodium borate

Preservative

0.05% Sodium Azide

Concentration

5.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Goat IgG Isotype Control [Janelia Fluor 549]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Goat IgG Isotype Control [Janelia Fluor 549]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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