Human IgG Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97043
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Human IgG
Format
Purified
Concentration
LYOPH mg/ml
Product Specifications
Host
Human
Isotype
IgG
Description
This product was prepared from normal human serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG and anti-Human Serum.
Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
Scientific Data Images for Human IgG Isotype Control
Western Blot: Human IgG Isotype Control [NBP1-97043]
Western Blot: Human IgG Isotype Control [NBP1-97043] - SDS-PAGE of Human IgG.Lane 1: Reduced Human IgG.Lane 2: 5uL OPAL Pre-stained MarkerLane 3: Non-reduced Human IgG.Load: 1ug per lane.Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.SDS-PAGE: Human IgG Isotype Control [NBP1-97043]
SDS-Page: Human IgG Isotype Control [NBP1-97043] - SDS-PAGE of Human IgG Isotype control.Lane 1: Reduced Human IgG Isotype control.Lane 2: 5uL OPAL Pre-stained Marker. Load: 1ug per lane.Predicted/Observed size: Non-reduced at 180-245 kDa , Reduced at 55, 25 kDa.Applications for Human IgG Isotype Control
Application
Recommended Usage
ELISA
1:10000 - 1:50000
Flow Cytometry
1:500
Western Blot
1:1000 - 1:5000
Application Notes
This isotype control can be utilized as a control or standard reagent in Western Blotting, Flow, and ELISA experiments.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for Human IgG Isotype Control
Product Specific Notices for Human IgG Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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