Sheep IgG Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97055
![SDS-PAGE: Sheep IgG Isotype Control [NBP1-97055]](https://resources.bio-techne.com/images/products/Sheep IgG Isotype Control-SDS-Page-NBP1-97055-img0005.jpg "SDS-PAGE: Sheep IgG Isotype Control [NBP1-97055]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Sheep
Applications
ELISA, Immunohistochemistry, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Sheep IgG
Format
Purified
Concentration
LYOPH mg/ml
Product Summary for Sheep IgG Isotype Control
Host
Sheep
Isotype
IgG
Description
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep IgG and anti-Sheep Serum.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Sheep IgG Isotype Control
SDS-PAGE: Sheep IgG Isotype Control [NBP1-97055]
SDS-Page: Sheep IgG Isotype Control [NBP1-97055] - Lane 1: Sheep IgG Non-Reduced Lane 2: Sheep IgG Reduced Load: 1.0 ug per lane Non-Reduced Predicted/Observed Size: 160 kDa/160 kDa Reduced Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa![SDS-PAGE: Sheep IgG Isotype Control [NBP1-97055]](https://resources.bio-techne.com/images/products/Sheep-IgG-Isotype-Control-SDS-Page-NBP1-97055-img0004.jpg "SDS-PAGE: Sheep IgG Isotype Control [NBP1-97055]")
SDS-PAGE: Sheep IgG Isotype Control [NBP1-97055]
SDS-Page: Sheep IgG Isotype Control [NBP1-97055] - SDS PAGE Results of Sheep IgG Isotype control. Lane 1: Sheep IgG Reduced [5ug]. Lane 2: Sheep IgG Reduced [1ug]. Lane 3: Opal Prestained Molecular Weight Marker. Lane 4: Sheep IgG Non-Reduced [1ug]. Lane 5: Sheep IgG Non-Reduced [5ug]. 4-20% Gel, Coomassie stained.Applications for Sheep IgG Isotype Control
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
SDS-Page
1:100 - 1:2000
Western Blot
1:100 - 1:2000
Application Notes
This product has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Purified
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for Sheep IgG Isotype Control
Product Specific Notices for Sheep IgG Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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