Rabbit IgG Isotype Control
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24891
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Rabbit
Applications
Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, In vitro assay
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Scientific Data Images for Rabbit IgG Isotype Control
Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891]
Immunohistochemistry: Rabbit IgG Isotype Control [NBP2-24891] - Controls for alpha-MSH immunohistochemistry and immunofluorescence. (A) Isotype controls for alpha-MSH were obtained by staining consecutive sections of human and mouse atherosclerotic plaques with alpha-MSH antibody or rabbit polyclonal isotype control antibody. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-33523-7) licensed under a CC-BY license.Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891]
Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.Applications for Rabbit IgG Isotype Control
Application
Recommended Usage
Chromatin Immunoprecipitation
1:10-1:500. Use reported in scientific literature (PMID 23034403)
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 32784470)
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10 - 1:500. Use reported in scientific literature (PMID 255994242)
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. In vitro assay reported in multiple scientific literatures with the Azide free version (NBP2-24893)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-24891 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IgG
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Gene Symbol
IGHG1
Additional IgG Products
Product Documents for Rabbit IgG Isotype Control
Product Specific Notices for Rabbit IgG Isotype Control
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
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