Mouse IgG2b Isotype Control (M2B)

Mouse

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, SDS-Page, Western Blot

Unconjugated

Monoclonal Mouse IgG_2B Clone # M2B

Purified

Please contact technical services for concentration.

Extensive cross-adsorption was performed to remove any unwanted subclasses. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM

Monoclonal

Mouse

IgG_2B

This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM. Confirmed by SDS-PAGE.

Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Protein A purified

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Purified

0.01% Sodium Azide

Please contact technical services for concentration.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.