Mouse IgG2A Isotype Control
R&D Systems, part of Bio-Techne | Catalog # MAB0031
Key Product Details
Species Reactivity
Mouse
Applications
Control
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 133304
Product Specifications
Immunogen
KLH
Specificity
Recognizes KLH (Keyhole Limpet Hemocyanin). In immunohistochemistry or flow cytometry of a variety of cell types or tissues, no background staining is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IgG2A Isotype Control
Detection of Mouse IgG Control by Flow Cytometry
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 Monoclonal Antibody (Catalog # MAB1298, filled histogram) or Mouse IgG Isotype Control Antibody (Catalog # MAB0031, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog F0101B).Applications for Mouse IgG2A Isotype Control
Application
Recommended Usage
Control
Negative control for flow cytometry, immunohistochemistry, or immunocytochemistry experiments. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB0031 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2A
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for Mouse IgG2A Isotype Control
Product Specific Notices for Mouse IgG2A Isotype Control
For research use only
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