Rat IgG2A Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC006F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
Control
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 54447
Product Specifications
Immunogen
KLH
Specificity
Selected for use as rat IgG2A isotype control for flow cytometry applications.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Rat IgG2A Fluorescein-conjugated Antibody
Detection of Rat IgG Control by Flow Cytometry
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB17582F, filled histogram) or Rat IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Rat IgG2A Fluorescein-conjugated Antibody
Application
Recommended Usage
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2A Fc
Additional IgG2A Products
Product Documents for Rat IgG2A Fluorescein-conjugated Antibody
Product Specific Notices for Rat IgG2A Fluorescein-conjugated Antibody
For research use only
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