Mouse splenocytes treated with 1 µg/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB168A, filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat IgG2B APC‑conjugated Isotype Control

Application

Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/10⁶ cells.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Rat IgG2B APC‑conjugated Isotype Control

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Rat IgG2B APC‑conjugated Isotype Control

For research use only

Citations
Reviews

Rat IgG2B APC‑conjugated Isotype Control

R&D Systems, part of Bio-Techne | Catalog # IC013A

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all IgG2B Isotype Controls »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat IgG2B APC‑conjugated Isotype Control

Detection of Rat IgG Control by Flow Cytometry