Rat IgG2B APC‑conjugated Isotype Control
R&D Systems, part of Bio-Techne | Catalog # IC013A
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Rat
Applications
Control
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 141945
Product Specifications
Immunogen
KLH
Specificity
Selected for use as a rat IgG2B isotype control for flow cytometry applications.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Rat IgG2B APC‑conjugated Isotype Control
Detection of Rat IgG Control by Flow Cytometry
Mouse splenocytes treated with 1 µg/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB168A, filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Rat IgG2B APC‑conjugated Isotype Control
Application
Recommended Usage
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2B
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2B Fc
Additional IgG2B Products
Product Documents for Rat IgG2B APC‑conjugated Isotype Control
Product Specific Notices for Rat IgG2B APC‑conjugated Isotype Control
For research use only
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