DC-SIGNR/CD299/CLEC4M Overexpression Lysate

Name: DC-SIGNR/CD299/CLEC4M Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-09263

Novus Biologicals, part of Bio-Techne | Catalog # NBL1-09263

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for DC-SIGNR/CD299/CLEC4M Overexpression Lysate

DC-SIGNR/CD299/CLEC4M Transient Overexpression Lysate
Expression Host: HEK293T

Plasmid: RC211716

Accession#: NM_214675

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

TMW

28.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for DC-SIGNR/CD299/CLEC4M Overexpression Lysate

Western Blot: DC-SIGNR/CD299/CLEC4M Overexpression Lysate [NBL1-09263]

Western Blot: CLEC4M Overexpression Lysate (Adult Normal) [NBL1-09263] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLEC4M.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: DC-SIGNR/CD299

This gene, often referred to as L-SIGN, is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, with a large impact on public health, ranging from parasites to viruses. The encoded protein is a trans-membrane C-type lectin with an extracellular portion composed of a tandem repeat region and a carbohydrate recognition domain. The extracellular region of the protein plays a critical role in pathogen recognition and binding. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835; often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. In addition, a structural difference has been observed in the extracellular tandem-repeat region which has a constant size in DC-SIGN and is polymorphic in L-SIGN. The tandem repeat region is important for protein homo-dimerization which required for ligand-binding. Studies of the variation in tandem repeat length suggest that the region may be a target of selective pressure by infectious pathogens. [provided by RefSeq]. Transcript Variant: This variant (2) lacks a segment in the 3' UTR, as compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 2

Alternate Names

CD209L, CD299, CLEC4M, DC-SIGN2, DCSIGNR, LSIGN

Gene Symbol

CLEC4M

Additional DC-SIGNR/CD299 Products

Product Documents for DC-SIGNR/CD299/CLEC4M Overexpression Lysate

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for DC-SIGNR/CD299/CLEC4M Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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