Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

Name: Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein
Brand: Novus Biologicals
SKU: NBP2-26580

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26580

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Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Conjugate

Unconjugated

Applications

Functional Assay, SDS-PAGE

View all 4-1BB Ligand/TNFSF9 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein corresponding to TNFSF9.

Amino Acid Sequence: EPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSRTPGKSGRTEPRPALTITTSPNLGTRENNADQVTPVSHIGCPNTTQQGSPVFAKLLAKNQASLCNTTLNWHSQDGAGSSYLSQGLRYEEDKKELVVDSPGLYYVFLELKLSPTFTNTGHKVQGWVSLVLQAKPQVDDFDNLALTVELFPCSMENKLVDRSWSQLLLLKAGHRLSVGLRAYLHGAQDAYRDWELSYPNTTSFGLFLVKPDNPWE
Mouse Ig-Fc amino acid sequences are in Italics
Linker sequence (SGR) in bold
Mouse CD137L sequences are underlined

Purity

Protein A purified

Endotoxin Level

Endotoxin level < 0.1 EU/ug of the protein (< 0.01 ng/ug of the protein) as determined by the LAL test.

Activity

In vitro proliferation assay, in vivo assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

Functional: Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein [NBP2-26580]

Functional: Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein [NBP2-26580] - Demonstration of the potency of mCD137L fusion protein in vitro in comparison to an agonist antibody 2A. Balb/c mouse splenocytes were incubated for 48 hrs on anti-CD3 (145-11C clone) monoclonal antibody coated plates in the presence of 2 mg/ml anti-CD3, 2A or Fc-mCD137L. The amount of IL-2 production induced by Fc-mCD137L was comparable to the induction by 2A antibody.

SDS-PAGE: Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein [NBP2-26580]

SDS-Page: Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein [NBP2-26580] - Electrophoretic analysis of the purified Fc-mCD137L using Coomassie Blue stained 4-20% reducing SDS-PAGE.

Formulation, Preparation and Storage

NBP2-26580

Formulation	Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 um filter sterilized.
Concentration	2.0 mg/ml
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: 4-1BB Ligand/TNFSF9

CD137L is a member of the TNF superfamily and provides costimulatory signals to T cells. It is expressed on dendritic cells, activated macrophages, and B cells. CD137L binds to its receptor CD137 on activated T cells, this interaction is important for adaptive T-cell response. The engagement of CD137 by CD137L provides costimulation to both CD4+ and CD8+ cells. Agonist anti-CD137 monoclonal antibodies also have been used to stimulate CD137 to regulate T-cell number and functions. The Fc-CD137L fusion protein was generated by cloning the extracellular domain of mouse CD137L with the Fc region of mouse immunoglobulin (hinge region, CH2, CH3 domains). The Fc-CD137L fusion protein has comparable biological activities as 2A, a CD137 agonist antibody.

Alternate Names

41BB Ligand, CD137L, TNFSF9

Gene Symbol

TNFSF9

Additional 4-1BB Ligand/TNFSF9 Products

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COA

Product Specific Notices for Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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