Recombinant Human ALDH2 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-19053

Novus Biologicals, part of Bio-Techne
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all ALDH2 Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids18-517 of Human ALDH2

Source: E.coli

Amino Acid Sequence: MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

54.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 250pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole Acetaldehyde by NAD per minute at pH 8.0 at 25C

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ALDH2 Protein

SDS-PAGE: Recombinant Human ALDH2 Protein [NBC1-19053]

SDS-PAGE: Recombinant Human ALDH2 Protein [NBC1-19053]

SDS-Page: Recombinant Human ALDH2 Protein [NBC1-19053] - Molecular Weight: 54.5 kDa (501aa) on 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBC1-19053
Formulation 20 mM Tris-HCl buffer (pH 7.5), 1 mM DTT, 1 mM EDTA, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ALDH2

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Aldehyde Dehydrogenase 2 Family Member

Alternate Names

ALDHI, ALDM

Gene Symbol

ALDH2

Additional ALDH2 Products

Product Documents for Recombinant Human ALDH2 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human ALDH2 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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