Recombinant Human Alix His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-40401

Novus Biologicals, part of Bio-Techne
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NBP1-40401
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Key Product Details

Source

E. coli

Accession #

Q8WUM4

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Alix Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-392 of Human Alix

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATFISVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR RAAVGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKIAA KHYQFASGAF LHIKETVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPVLAAKHC IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKTVASR YDEYVNVKDF SDKINRALAA AKKDNDFIYH DRVPDLKDLD PIGKATLVKS TPVNVPISQK FTDLFEKMVP VSVQQSLAAY NQRKADLVNR SIAQMREATT LA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

45.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Alix His Protein

SDS-PAGE: Recombinant Human Alix His Protein [NBP1-40401]

SDS-PAGE: Recombinant Human Alix His Protein [NBP1-40401]

SDS-Page: Recombinant Human Alix Protein [NBP1-40401] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation and Storage

NBP1-40401
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alix

Alix, also known as PDCD6IP, is a cytoplasmic protein that interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. It's involved in concentration and sorting of cargo proteins of the multivesicular body for incorporation into intralumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. Overexpression of this protein and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Recombinant PDCD6IP protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

AIP1DRIP4, ALG-2 interacting protein 1, ALG-2 interacting protein X, ALG-2-interacting protein 1, alinx, ALIX, apoptosis-linked gene 2-interacting protein X, dopamine receptor interacting protein 4, Hp95, KIAA1375, MGC17003, PDCD6-interacting protein, programmed cell death 6 interacting protein, programmed cell death 6-interacting protein

Gene Symbol

PDCD6IP

UniProt

Q8WUM4 (Human)

Additional Alix Products

Product Documents for Recombinant Human Alix His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Alix His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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