Recombinant Human Amphiregulin Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35055
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 98 amino acid residues corresponding to Amphiregulin Source: E. coli
Uniprot ID: P15514
Amino Acid Sequence: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of Amphiregulin as determined by LAL method.
Predicted Molecular Mass
11.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Amphiregulin Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is between 5-10 ng/ml.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Amphiregulin Protein
![SDS-PAGE: Recombinant Human Amphiregulin Protein [NBP2-35055]](https://resources.bio-techne.com/images/products/Human-Amphiregulin-Protein-SDS-Page-NBP2-35055-img0001.jpg "SDS-PAGE: Recombinant Human Amphiregulin Protein [NBP2-35055]")
SDS-PAGE: Recombinant Human Amphiregulin Protein [NBP2-35055]
SDS-Page: Recombinant Human Amphiregulin Protein [NBP2-35055]Formulation, Preparation and Storage
NBP2-35055
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Amphiregulin
Additional Amphiregulin Products
Product Documents for Recombinant Human Amphiregulin Protein
Product Specific Notices for Recombinant Human Amphiregulin Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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