Recombinant Human AMPK beta 2/PRKAB2 His Protein

Name: Recombinant Human AMPK beta 2/PRKAB2 His Protein
Brand: Novus Biologicals
SKU: NBP2-23376

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23376

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all AMPK beta 2 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-272 of Human AMPK beta 2/PRKAB2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGNTTS DRVSGERHGA KAARSEGAGG HAPGKEHKIM VGSTDDPSVF SLPDSKLPGD KEFVSWQQDL EDSVKPTQQA RPTVIRWSEG GKEVFISGSF NNWSTKIPLI KSHNDFVAIL DLPEGEHQYK FFVDGQWVHD PSEPVVTSQL GTINNLIHVK KSDFEVFDAL KLDSMESSET SCRDLSSSPP GPYGQEMYAF RSEERFKSPP ILPPHLLQVI LNKDTNISCD PALLPEPNHV MLNHLYALSI KDSVMVLSAT HRYKKKYVTT LLYKPI

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human AMPK beta 2/PRKAB2 His Protein

SDS-PAGE: Recombinant Human AMPK beta 2/PRKAB2 His Protein [NBP2-23376]

SDS-Page: Recombinant Human AMPK beta 2/PRKAB2 Protein [NBP2-23376]

Formulation, Preparation and Storage

NBP2-23376

Formulation	20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 2M UREA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AMPK beta 2

PRKAB2 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. PRKAB2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Recombinant human PRKAB2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

AMP-activated Protein Kinase beta 2

Alternate Names

PRKAB2

Gene Symbol