Recombinant Mouse APRIL/TNFSF13 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35145
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 146 amino acids corresponding to APRIL/TNFSF13 Source: E. coli
Uniprot ID: Q9D777
Amino Acid Sequence: AVLTQKHKKK HSVLHLVPVN ITSKADSDVT EVMWQPVLRR GRGLEAQGDI VRVWDTGIYL LYSQVLFHDV TFTMGQVVSR EGQGRRETLF RCIRSMPSDP DRAYNSCYSA GVFHLHQGDI ITVKIPRANA KLSLSPHGTF LGFVKL
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of APRIL/TNFSF13 as determined by LAL method.
Predicted Molecular Mass
16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
TNFSF13 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using activated T cells.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-35145
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: APRIL/TNFSF13
Long Name
A Proliferation-inducing Ligand
Alternate Names
CD256, TALL2, TNFSF13, TRDL1, ZTNF2, sAPRIL, sAPRIL/TNFSF13
Gene Symbol
TNFSF13
Additional APRIL/TNFSF13 Products
Product Documents for Recombinant Mouse APRIL/TNFSF13 Protein
Product Specific Notices for Recombinant Mouse APRIL/TNFSF13 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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