Recombinant Human BAFF/BLyS/TNFSF13B Protein

Name: Recombinant Human BAFF/BLyS/TNFSF13B Protein
Brand: Novus Biologicals
SKU: NBP2-34883

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34883

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

Q9Y275

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all BAFF/BLyS/TNFSF13B Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to BAFF/BLyS/TNFSF13B Source: E. coli

Uniprot ID: Q9Y275

Amino Acid Sequence: MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of BAFF/BLyS/TNFSF13B as determined by LAL method.

Predicted Molecular Mass

17.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a mouse splenocyte survival assay is 0.5-2 ug/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human BAFF/BLyS/TNFSF13B Protein

SDS-PAGE: Recombinant Human BAFF/BLyS/TNFSF13B Protein [NBP2-34883]

SDS-Page: Recombinant Human BAFF/BLyS/TNFSF13B Protein [NBP2-34883]

Formulation, Preparation and Storage

NBP2-34883

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: BAFF/BLyS/TNFSF13B

BAFF, also known as BLyS and TALL-1, is a 32 kDa TNF family transmembrane protein that plays a critical role in the development and survival of B lineage cells. BAFF also costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells. It additionally promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages. It can be expressed as a homotrimer or as a heteromer in association with APRIL. BAFF is produced by monocytes, macrophages, neutrophils, dendritic cells, T cells, adipocytes. BAFF signals through the receptors BCMA, TACI, and BAFF R which are primarily expressed by B cells.

Long Name

B cell Activating Factor

Alternate Names

BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4, BAFF/TNFSF20

Gene Symbol

TNFSF13B

UniProt