Recombinant Human beta-Defensin 3 Protein

Name: Recombinant Human beta-Defensin 3 Protein
Brand: Novus Biologicals
SKU: NBP2-34913

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34913

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P81534

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all beta-Defensin 3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 45 amino acids corresponding to beta-Defensin 3 Source: E. coli

Uniprot ID: P81534

Amino Acid Sequence: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of beta-Defensin 3 as determined by LAL method.

Predicted Molecular Mass

5.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 ug/ml, corresponding to a specific activity of > 33.3 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human beta-Defensin 3 Protein

SDS-PAGE: Recombinant Human beta-Defensin 3 Protein [NBP2-34913]

SDS-Page: Recombinant Human beta-Defensin 3 Protein [NBP2-34913]

Formulation, Preparation and Storage

NBP2-34913

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: beta-Defensin 3

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 k Da proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. B-Defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Four human B-defensins have been identified and they are expressed on some leukocytes and at epithelial surfaces. Because B-defensins is cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca 2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane.

Alternate Names

BD14, BD3, betaDefensin 3, DEFB103A, DEFB103B, DEFB3, HBD-3

Gene Symbol

DEFB103B

UniProt