Recombinant Mouse Betacellulin/BTC Protein

Name: Recombinant Mouse Betacellulin/BTC Protein
Brand: Novus Biologicals
SKU: NBP2-35148

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35148

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Product Specifications
Scientific Data
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Background
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Key Product Details

Source

E. coli

Accession #

Q05928

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Betacellulin/BTC Proteins and Enzymes »

Product Specifications

Description

A recombinant protein containing 80 amino residues corresponding to Betacellulin/BTC, which comprises the mature EGF homologous portion of the Betacellulin protein Source: E. coli

Uniprot ID: Q05928

Amino Acid Sequence: DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYCIHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Betacellulin/BTC as determined by LAL method.

Predicted Molecular Mass

9.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Betacellulin/BTC protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 x 10^8 IU/mg

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Betacellulin/BTC Protein

SDS-PAGE: Recombinant Mouse Betacellulin/BTC Protein [NBP2-35148]

SDS-Page: Mouse Betacellulin/BTC Protein [NBP2-35148]

Formulation, Preparation and Storage

NBP2-35148

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Betacellulin/BTC

Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80 % identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant murine Betacellulin is a heparin binding protein containing 80 amino acids residues.

Alternate Names

BTC

Gene Symbol

BTC

UniProt