Recombinant Human BMP-4 Protein

Name: Recombinant Human BMP-4 Protein
Brand: Novus Biologicals
SKU: NBP2-34931

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34931

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P12644

Conjugate

Unconjugated

Applications

ELISA, SDS-PAGE, Western Blot

View all BMP-4 Proteins and Enzymes »

Product Specifications

Description

A monomeric, non-glycosylated polypeptide chain containing 117 amino acids corresponding to BMP-4 Source: E. coli

Uniprot ID: P12644

Amino Acid Sequence: MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR

Purity

>95%, by SDS-PAGE

Endotoxin Level

Less than 1 EU/ug of BMP-4 as determined by LAL method.

Predicted Molecular Mass

13.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

1. Molecular standard (Western, ELISA) in studying secreted BMP-4;
2. Preparing antibodies for BMP-4 monomer;
3. Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human BMP-4 Protein

SDS-PAGE: Recombinant Human BMP-4 Protein [NBP2-34931]

SDS-Page: Human BMP-4 Protein [NBP2-34931]

Formulation, Preparation and Storage

NBP2-34931

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 50 mM Na2CO3, 5 mM DTT, 0.02% Tween 20, pH 11.0.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water to a concentration of 0.1-1.0mg/mL
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: BMP-4

BMP-4 plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Mature BMP-4 forms homodimers and heterodimers with BMP-7. BMP-4/BMP-7 heterodimers show greater potency at osteogenic differentiation compared to BMP-4 homodimers. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.

Long Name

Bone Morphogenetic Protein 4

Alternate Names

BMP2B, BMP2B1, BMP4, MCOPS6, OFC11

Gene Symbol

BMP4

UniProt