Would you please help confirm if NBP1-97058 can be used as the blocking agent for applications listed on its datasheet?

Name: Bovine Serum Albumin Native Protein
Brand: Novus Biologicals
SKU: NBP1-97058

Yes, this BSA could be used to block, however, for most applications, this is not nearly enough BSA to use for blocking. This is intended to be run on a gel or used as a positive control.

Bovine Serum Albumin Native Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97058

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Conjugate

Unconjugated

View all Bovine Serum Albumin Proteins and Enzymes »

Product Specifications

Description

Native Bovine Bovine Serum Albumin

Source: Normal serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Purity

Multi-step

Application Notes

This product is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding.

Protein / Peptide Type

Native Protein

Formulation, Preparation and Storage

NBP1-97058

Preparation Method	This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and selective precipitation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Albumin and anti-Bovine Serum.
Formulation	Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	0.01% Sodium Azide
Concentration	Lyoph
Reconstitution	Reconstitute with 1.0 ml deionized water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C.

Background: Bovine Serum Albumin

Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screening assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA.

Alternate Names

albumin, BSA, cell growth inhibiting protein 42, DKFZp779N1935, growth-inhibiting protein 20, PRO0883, PRO0903, PRO1341, serum albumin

Gene Symbol

ALB

Additional Bovine Serum Albumin Products

Product Documents for Bovine Serum Albumin Native Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Bovine Serum Albumin Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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