Recombinant Human Cardiotrophin-1/CT-1 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61364
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 201 amino acids corresponding to Cardiotrophin-1/CT-1 Source: E. coli
Uniprot ID: Q16619
Amino Acid Sequence: MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of Cardiotrophin-1/CT-1 as determined by LAL method.
Predicted Molecular Mass
21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Cardiotrophin-1/CT-1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0x 10^6 IU/mg.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-61364
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: Cardiotrophin-1/CT-1
Alternate Names
Cardiotrophin1, CTF1
Gene Symbol
CTF1
Additional Cardiotrophin-1/CT-1 Products
Product Documents for Recombinant Human Cardiotrophin-1/CT-1 Protein
Product Specific Notices for Recombinant Human Cardiotrophin-1/CT-1 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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