Recombinant Mouse Cardiotrophin-1/CT-1 Protein

Name: Recombinant Mouse Cardiotrophin-1/CT-1 Protein
Brand: Novus Biologicals
SKU: NBP2-35170

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35170

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q60753

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Cardiotrophin-1/CT-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 203 amino acids corresponding to Cardiotrophin-1/CT-1 Source: E. coli

Uniprot ID: Q60753

Amino Acid Sequence: MSQREGSLED HQTDSSISFL PHLEAKIRQT HNLARLLTKY AEQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSVLPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP GPEPVTVATL FTANSTAGIF SAKVLGFHVC GLYGEWVSRT EGDLGQLVPG GVA

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Cardiotrophin-1/CT-1 as determined by LAL method.

Predicted Molecular Mass

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Cardiotrophin-1/CT-1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Cardiotrophin-1/CT-1 Protein

SDS-PAGE: Recombinant Mouse Cardiotrophin-1/CT-1 Protein [NBP2-35170]

SDS-Page: Mouse Cardiotrophin-1/CT-1 Protein [NBP2-35170]

Formulation, Preparation and Storage

NBP2-35170

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Cardiotrophin-1/CT-1

Cardiotrophin1 (CT1) is a member of the cytokine family which also includes IL6, IL11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. In addition, CT1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Murine CT1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide. Human and murine CT1 share 80 % a.a. sequence identity and exhibit crossspecies activity.

Alternate Names

Cardiotrophin1, CTF1

Gene Symbol

CTF1

UniProt