Recombinant Human Catalase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98907

Novus Biologicals, part of Bio-Techne
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NBP1-98907-0.5mg
NBP1-98907-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Catalase Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-527 of Human Catalase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MADSRDPASD QMQHWKEQRA AQKADVLTTG AGNPVGDKLN VITVGPRGPL LVQDVVFTDE MAHFDRERIP ERVVHAKGAG AFGYFEVTHD ITKYSKAKVF EHIGKKTPIA VRFSTVAGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDPIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HMNGYGSHTF KLVNANGEAV YCKFHYKTDQ GIKNLSVEDA ARLSQEDPDY GIRDLFNAIA TGKYPSWTFY IQVMTFNQAE TFPFNPFDLT KVWPHKDYPL IPVGKLVLNR NPVNYFAEVE QIAFDPSNMP PGIEASPDKM LQGRLFAYPD THRHRLGPNY LHIPVNCPYR ARVANYQRDG PMCMQDNQGG APNYYPNSFG APEQQPSALE HSIQYSGEVR RFNTANDDNV TQVRAFYVNV LNEEQRKRLC ENIAGHLKDA QIFIQKKAVK NFTEVHPDYG SHIQALLDKY NAEKPKNAIH TFVQSGSHLA AREKANL

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

61.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 100,000 units/mg. One unit will decompose 1.0umole of H2O2 per minute at pH 8.0 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Catalase His Protein

Recombinant Human Catalase Protein [NBP1-98907]

Formulation, Preparation and Storage

NBP1-98907
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Catalase

CAT is a key antioxidant enzyme in the bodies defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Recombinant human CAT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Cas1, CAT, Cs-1

Gene Symbol

CAT

Additional Catalase Products

Product Documents for Recombinant Human Catalase His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Catalase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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