Recombinant Human Cathepsin F His Protein

Name: Recombinant Human Cathepsin F His Protein
Brand: Novus Biologicals
SKU: NBP2-22739

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22739

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Cathepsin F Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 271-484 of Human Cathepsin F

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSAPPEWDW RSKGAVTKVK DQGMCGSCWA FSVTGNVEGQ WFLNQGTLLS LSEQELLDCD KMDKACMGGL PSNAYSAIKN LGGLETEDDY SYQGHMQSCN FSAEKAKVYI NDSVELSQNE QKLAAWLAKR GPISVAINAF GMQFYRHGIS RPLRPLCSPW LIDHAVLLVG YGNRSDVPFW AIKNSWGTDW GEKGYYYLHR GSGACGVNTM ASSAVVD

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Cathepsin F His Protein

SDS-PAGE: Recombinant Human Cathepsin F His Protein [NBP2-22739]

SDS-Page: Recombinant Human Cathepsin F Protein [NBP2-22739]

Formulation, Preparation and Storage

NBP2-22739

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin F

Cathepsin F Protein, also known as CTSF, belongs to the cathepsin family. Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. The CTSF gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. CTSF is thought to play a role in normal protein catabolism, and because it is highly expressed in some cancer cell lines, it may be involved in degradative processes occurring during tumor progression. Recombinant human CTSF protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

CTSF

Gene Symbol