Recombinant Mouse CCL11/Eotaxin Protein

Name: Recombinant Mouse CCL11/Eotaxin Protein
Brand: Novus Biologicals
SKU: NBP2-35177

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35177

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P48298

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL11/Eotaxin Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL11/Eotaxin Source: E. coli

Uniprot ID: P48298

Amino Acid Sequence: HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL11/Eotaxin as determined by LAL method.

Predicted Molecular Mass

8.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Eotaxin Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL11/Eotaxin Protein

SDS-PAGE: Recombinant Mouse CCL11/Eotaxin Protein [NBP2-35177]

SDS-Page: Mouse, CCL11/Eotaxin Protein [NBP2-35177]

Formulation, Preparation and Storage

NBP2-35177

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL11/Eotaxin

Murine CCL11 is belonging to the CC chemokine family. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Murine CCL11 is approximately 63 % identical at the amino acid level to human CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor

Alternate Names

Eotaxin, CCL11, Eotaxin/CCL11

Gene Symbol

CCL11

UniProt