Recombinant Mouse CCL12/MCP-5 Protein

Name: Recombinant Mouse CCL12/MCP-5 Protein
Brand: Novus Biologicals
SKU: NBP2-35163

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35163

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Product Specifications
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

Q62401

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL12/MCP-5 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 82 amino acids corresponding to CCL12/MCP-5 Source: E. coli

Uniprot ID: Q62401

Amino Acid Sequence: GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL12/MCP-5 as determined by LAL method.

Predicted Molecular Mass

9.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL12/MCP-5 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35163

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL12/MCP-5

Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2.

Alternate Names

MCP-5, MCP5, MCP-5/CCL12

Gene Symbol

Ccl12

UniProt