Recombinant Human CCL15/MIP-1 delta Protein

Name: Recombinant Human CCL15/MIP-1 delta Protein
Brand: Novus Biologicals
SKU: NBP2-35043

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35043

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q16663

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL15/MIP-1 delta Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 68 amino acids corresponding to CCL15/MIP-1 delta Source: E. coli

Uniprot ID: Q16663

Amino Acid Sequence: SFHFAADCCT SYISQSIPCS LMKSYFETSS ECSKPGVIFL TKKGRQVCAK PSGPGVQDCM KKLKPYSI

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CCL15/MIP-1 delta as determined by LAL method.

Predicted Molecular Mass

7.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL15/MIP-1 delta protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL15/MIP-1 delta Protein

SDS-PAGE: Recombinant Human CCL15/MIP-1 delta Protein [NBP2-35043]

SDS-Page: Human CCL15/MIP-1 delta 68 a.a Protein [NBP2-35043]

Formulation, Preparation and Storage

NBP2-35043

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.2.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL15/MIP-1 delta

Human CCL15 is belonging to the CC chemokine family and shares 35 % amino acid homology with human HCC1 (CCL14). CCL15 is most abundant in heart, skeletal muscle and adrenal gland, and low expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. CCL15 has several cleaved chains. All of them are more potent chemoattractants than CCL15.

Alternate Names

HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3, CCL15, MIP-5/CCL15

Gene Symbol

CCL15

UniProt