Recombinant Human CCL21/6Ckine Protein

Name: Recombinant Human CCL21/6Ckine Protein
Brand: Novus Biologicals
SKU: NBP2-35002

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35002

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

O00585

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL21/6Ckine Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 111 amino acids corresponding to CCL21/6Ckine Source: E. coli

Uniprot ID: O00585

Amino Acid Sequence: SDGGAQDCCL KYSQRKIPAK VVRSYRKQEP SLGCSIPAIL FLPRKRSQAE LCADPKELWV QQLMQHLDKT PSPQKPAQGC RKDRGASKTG KKGKGSKGCK RTERSQTPKG P

Purity

>95%, by SDS-PAGE

Endotoxin Level

Less than 1 EU/ug of CCL21/6Ckine as determined by LAL method.

Predicted Molecular Mass

12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL21/6Ckine Protein

SDS-PAGE: Recombinant Human CCL21/6Ckine Protein [NBP2-35002]

SDS-Page: Human CCL21/6Ckine Protein [NBP2-35002]

Formulation, Preparation and Storage

NBP2-35002

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2x PBS, pH 7.0.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL21/6Ckine

CCL21 is encoded by CCL21 gene that is located in Chr. 9 and shows 21-33 % identity to other CC chemokines. This chemokine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxylterminal domain. CCL21 is highly expressed in lymph nodes of certain endothelial cells, and in the spleen and appendix. It elicits effects by binding with CCR7, having functions of T and B lymphocytes chemotaxis and inhibiting hematopoiesis.

Alternate Names

6Ckine, exodus-2, SCYA21, SLC, TCA-4, Exodus-2/CCL21

Gene Symbol

CCL21

UniProt