Recombinant Human CCL22/MDC Protein

Name: Recombinant Human CCL22/MDC Protein
Brand: Novus Biologicals
SKU: NBP2-34977

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34977

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

O00626

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL22/MDC Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 69 amino acids corresponding to CCL22/MDC Source: E. coli

Uniprot ID: O00626

Amino Acid Sequence: GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL22/MDC as determined by LAL method.

Predicted Molecular Mass

8.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

MDC Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL22/MDC Protein

SDS-PAGE: Recombinant Human CCL22/MDC Protein [NBP2-34977]

SDS-Page: Human CCL22/MDC Protein [NBP2-34977]

Formulation, Preparation and Storage

NBP2-34977

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH7.4, 500 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL22/MDC

CCL22 is a protein that in humans is encoded by the CCL22 gene, which locates on the Chr. 16. The protein is highly expressed in macrophage, monocyte-derived dendritic cell and thymus, additionally, also detected in the tissues of thymus, lymph node and appendix. CCL22 can bind to CCR4, and is a chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells, but not for neutrophils, eosinophils, and resting T-lymphocytes. After secreted from monocyte-derived dendritic cells, the protein can be proteolytic cleaved into three forms: MDC (3-69), MDC (5-69), MDC (7-69).

Alternate Names

ABCD-1, MDC, STCP-1, CCL22, MDC/CCL22

Gene Symbol

CCL22

UniProt