Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 Protein

Name: Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 Protein
Brand: Novus Biologicals
SKU: NBP2-35178

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35178

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

Q9JKC0

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL24/Eotaxin-2/MPIF-2 Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 93 amino acids corresponding to CCL24/Eotaxin-2/MPIF-2 Source: E. coli

Uniprot ID: Q9JKC0

Amino Acid Sequence: VTIPSSCCTS FISKKIPENR VVSYQLANGS ICPKAGVIFI TKKGHKICTD PKLLWVQRHI QKLDAKKNQP SKGAKAVRTK FAVQRRRGNS TEV

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL24/Eotaxin-2/MPIF-2 as determined by LAL method.

Predicted Molecular Mass

10.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Eotaxin 2 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35178

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL24/Eotaxin-2/MPIF-2

CCL24, also named MPIF-2, Eotaxin-2 and Ckb6, is a novel CC chemokine recently identified. It is a secreted protein, encoded by CCL24 gene, and produced by activated monocytes and T lymphocytes. CCL24 signals through the CCR3 receptor and has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.

Alternate Names

Eotaxin-2, MPIF-2, MPIF2, CCL24, Eotaxin-2/CCL24

Gene Symbol

CCL24

UniProt