Recombinant Mouse CCL3/MIP-1 alpha Protein

Name: Recombinant Mouse CCL3/MIP-1 alpha Protein
Brand: Novus Biologicals
SKU: NBP2-35193

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35193

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

P10855

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL3/MIP-1 alpha Proteins and Enzymes »

Product Specifications

Description

A recombinant protein containing 69 amino acid residues corresponding to CCL3/MIP-1 alpha, including the four highly conserved cysteine residues present in CC chemokines Source: E. coli

Uniprot ID: P10855

Amino Acid Sequence: APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CCL3/MIP-1 alpha as determined by LAL method.

Predicted Molecular Mass

7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL3 Protein is fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using murine splenocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL3/MIP-1 alpha Protein

SDS-PAGE: Recombinant Mouse CCL3/MIP-1 alpha Protein [NBP2-35193]

SDS-Page: Mouse CCL3/MIP-1 alpha Protein [NBP2-35193]

Formulation, Preparation and Storage

NBP2-35193

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1alpha can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant murine MIP-1alpha is a 7.9 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and mature mouse CCL3 shares 73 %, 91 % and 82 % amino acid sequence identity with human, rat and cotton rat CCL3, respectively.

Alternate Names

G0S19-1, LD78a, MIP-1 alpha, MIP1 alpha, MIP1A, PAT 464.1, SCYA3, SIS-beta, MIP-1 alpha/CCL3

Gene Symbol

CCL3

UniProt