Recombinant Rat CCL4/MIP-1 beta Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35278
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 69 amino acids corresponding to CCL4/MIP-1 beta Source: E. coli
Uniprot ID: P50230
Amino Acid Sequence: APIGSDPPTS CCFSYTSRKI HRNFVMDYYE TSSLCSQPAV VFLTKKGRQI CADPSEPWVN EYVNDLELN
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of CCL4/MIP-1 beta as determined by LAL method.
Predicted Molecular Mass
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
CCL4 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-1000 ng/ml.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-35278
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, 3 % trehalose. |
| Preservative | Trehalose |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: CCL4/MIP-1 beta
Additional CCL4/MIP-1 beta Products
Product Documents for Recombinant Rat CCL4/MIP-1 beta Protein
Product Specific Notices for Recombinant Rat CCL4/MIP-1 beta Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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