Recombinant Rhesus Macaque CCL5/RANTES Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61346

Novus Biologicals, part of Bio-Techne
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NBP2-61346-100ug
NBP2-61346-1mg
NBP2-61346-500ug
NBP2-61346-5ug
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

View all CCL5/RANTES Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 68 amino acids corresponding to CCL5/RANTES Source: E. coli

Amino Acid Sequence: SPHASDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CCL5/RANTES as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61346
Formulation Lyophilized from a 0.2 um filtered solution in 20mM PB, pH 6.0, 500mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL5/RANTES

RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5 (1). This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1 (2). CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites (3).

Alternate Names

RANTES, SISd

Gene Symbol

CCL5

Additional CCL5/RANTES Products

Product Documents for Recombinant Rhesus Macaque CCL5/RANTES Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Rhesus Macaque CCL5/RANTES Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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