Recombinant Mouse CCL6/C10 Protein

Name: Recombinant Mouse CCL6/C10 Protein
Brand: Novus Biologicals
SKU: NBP2-35158

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35158

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Product Datasheet / COA

Catalog #

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P27784

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL6/C10 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 95 amino acids corresponding to CCL6/C10 Source: E. coli

Uniprot ID: P27784

Amino Acid Sequence: GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL6/C10 as determined by LAL method.

Predicted Molecular Mass

10.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL6/C10 protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35158

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris, pH 8.0, 500 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL6/C10

Mouse C10, a member of the CC subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homolog for mouse C10 has not been identified. The expression of C10 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation.

Alternate Names

C10, MRP-1, SCYA6

Gene Symbol

Ccl6

UniProt