Recombinant Human CCL7/MCP-3/MARC Protein

Name: Recombinant Human CCL7/MCP-3/MARC Protein
Brand: Novus Biologicals
SKU: NBP2-34972

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34972

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Product Specifications
Scientific Data
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Key Product Details

Source

E. coli

Accession #

P80098

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL7/MCP-3/MARC Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 76 amino acids corresponding to CCL7/MCP-3/MARC Source: E. coli

Uniprot ID: P80098

Amino Acid Sequence: QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL7/MCP-3/MARC as determined by LAL method.

Predicted Molecular Mass

9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

MCP3 protein is fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL7/MCP-3/MARC Protein

SDS-PAGE: Recombinant Human CCL7/MCP-3/MARC Protein [NBP2-34972]

SDS-Page: Human CCL7/MCP-3/MARC Protein [NBP2-34972]

Formulation, Preparation and Storage

NBP2-34972

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL7/MCP-3/MARC

Human CCL7, also known as monocyte chemotactic protein 3 (MCP-3), is belonging to the CC chemokine family. MCP-3 has two homogeneous MCP-1 (CCL2) and MCP-2 (CCL8). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL7 shares 71 % and 58 % a.a. sequence identity with MCP-1 and MCP-2, respectively. CCL7 has chemotactic function for monocytes and eosinophils, but not for neutrophils. In addition, it can also chemoattract activated NK cells.

Alternate Names

MARC, MCP-3, CCL7, MCP-3/CCL7

Gene Symbol

CCL7

UniProt