Recombinant Human CD-M6PR His Protein

Name: Recombinant Human CD-M6PR His Protein
Brand: Novus Biologicals
SKU: NBP2-51688

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51688

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Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all CD-M6PR Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 27-185 of Human CD-M6PR

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSTEEKTCD LVGEKGKESE KELALVKRLK PLFNKSFEST VGQGSDTYIY IFRVCREAGN HTSGAGLVQI NKSNGKETVV GRLNETHIFN GSNWIMLIYK GGDEYDNHCG KEQRRAVVMI SCNRHTLADN FNPVSEERGK VQDCFYLFEM DSSLACSPEI SH

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

20.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CD-M6PR His Protein

SDS-PAGE: Recombinant Human CD-M6PR His Protein [NBP2-51688]

SDS-Page: Recombinant Human CD-M6PR Protein [NBP2-51688] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-51688

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD-M6PR

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and ~10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.

Long Name

Cation Dependent Mannose-6-Phosphate Receptor 46 kDa

Alternate Names

CD-MPR, CDM6PR, M6PR, MPR46, MPRD, SMPR

Gene Symbol

M6PR

Additional CD-M6PR Products

Product Documents for Recombinant Human CD-M6PR His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human CD-M6PR His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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