Recombinant Human CD23/Fc epsilon RII Protein

Name: Recombinant Human CD23/Fc epsilon RII Protein
Brand: Novus Biologicals
SKU: NBP2-35242

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35242

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P06734

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CD23/Fc epsilon RII Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 172 amino acids corresponding to CD23/Fc epsilon RII Source: E. coli

Uniprot ID: P06734

Amino Acid Sequence: MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CD23/Fc epsilon RII as determined by LAL method.

Predicted Molecular Mass

19.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CD23 Protein is fully biologically active when compared to standard. The ED50 determined by its ability to induce TNF-alpha production by human PBMCs.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CD23/Fc epsilon RII Protein

SDS-Page: Recombinant Human CD23/Fc epsilon RII Protein [NBP2-35242]

Formulation, Preparation and Storage

NBP2-35242

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CD23/Fc epsilon RII

CD23 is a type 2 transmembrane C-type lectin that binds IgE, CD21, CD11b and CD11c. It is expressed on a variety of hematopoietic cells and functions in allergic immune responses. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.

Long Name

Fc epsilon Receptor II

Alternate Names

CD23, CLEC4J, Fc epsilon RII, FCER2, Fcer2a, FceRII, IGEBF, Ly-42

Gene Symbol

FCER2

UniProt