I have a question about recombinant Human CD3 epsilon Protein (Cat # NBP2-22752: do you have gel images from non-reducing gel? Does the protein solution contain aggregates?

Name: Recombinant Human CD3 epsilon His Protein
Brand: Novus Biologicals
SKU: NBP2-22752
Rating: 4 (1 reviews)

We do not have an image in non-reducing conditions. As for aggregation, it has a hydrophobic region, so it may have some aggregation when boiled. Therefore, we recommend heating to 37C rather than boiling when loading.

Recombinant Human CD3 epsilon His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22752

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all CD3 epsilon Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 23-126 of Human CD3 epsilon

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSDGNEEMG GITQTPYKVS ISGTTVILTC PQYPGSEILW QHNDKNIGGD EDDKNIGSDE DHLSLKEFSE LEQSGYYVCY PRGSKPEDAN FYLYLRARVC ENCMEMD

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

14.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 4 using NBP2-22752 in the following applications:

Binding assay/Protein-protein interaction (1 Review)

Scientific Data Images for Recombinant Human CD3 epsilon His Protein

SDS-PAGE: Recombinant Human CD3 epsilon His Protein [NBP2-22752]

SDS-Page: Recombinant Human CD3 epsilon His Protein [NBP2-22752] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP2-22752

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 epsilon

CD3E is the CD3-epsilonpolypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. Recombinant human CD3E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for Recombinant Human CD3 epsilon His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human CD3 epsilon His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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