Recombinant Human Citidine Deaminase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49306

Novus Biologicals, part of Bio-Techne
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NBP1-49306-0.1mg
NBP1-49306-0.5mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Citidine Deaminase Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-146 of Human Citidine Deaminase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

18.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of required to deaminate 1.0 pmole of cytidine per min at pH 7.5 at 25C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Citidine Deaminase His Protein

SDS-PAGE: Recombinant Human Citidine Deaminase His Protein [NBP1-49306]

SDS-PAGE: Recombinant Human Citidine Deaminase His Protein [NBP1-49306]

SDS-Page: Recombinant Human Citidine Deaminase Protein [NBP1-49306] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-49306
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 2 mM EDTA, 100 mM NaCl, 40% Glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Citidine Deaminase

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Cytidine deaminase

Alternate Names

CDA, CDD, EC 3.5.4.5

Gene Symbol

CDA

Additional Citidine Deaminase Products

Product Documents for Recombinant Human Citidine Deaminase His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Citidine Deaminase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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