Recombinant Human CREB GST (N-Term) Protein

Name: Recombinant Human CREB GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00001385-P01
Rating: 4 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # H00001385-P01

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Citations (4)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

ELISA, Affinity Purification, Immunoprecipitation, Functional Assay, Microarray, Western Blot

View all CREB Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-341 of Human CREB1

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNHVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

63.25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Application Notes

Use in In vitro assay reported in scientific literature (PMID 24550008

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 4 using H00001385-P01 in the following applications:

In vitro kinase assay (1 Review)

Scientific Data Images for Recombinant Human CREB GST (N-Term) Protein

SDS-PAGE: Recombinant Human CREB GST (N-Term) Protein [H00001385-P01]

SDS-Page: CREB Recombinant Protein [H00001385-P01]

Formulation, Preparation and Storage

H00001385-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: CREB

CREB1( AAH10636, 1 a.a. - 342 a.a.) recombinant protein with GST.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Gene Symbol