Recombinant Human CXCL12/SDF-1 alpha Protein

Name: Recombinant Human CXCL12/SDF-1 alpha Protein
Brand: Novus Biologicals
SKU: NBP2-35258

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35258

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Product Datasheet / COA

Catalog #

Availability

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P48061

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL12/SDF-1 alpha Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 68 amino acids corresponding to CXCL12/SDF-1 alpha Source: E. coli

Uniprot ID: P48061

Amino Acid Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL12/SDF-1 alpha as determined by LAL method.

Predicted Molecular Mass

8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

SDF1 alpha Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35258

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL12/SDF-1 alpha

CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1 beta, SDF-1 alpha is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.

Alternate Names

SDF1 alpha, SDF1a, SDF-1 alpha, SDF-1 alpha/CXCL12 alpha

Gene Symbol

CXCL12

UniProt