Recombinant Rat CXCL12/SDF-1 beta Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35291
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 72 amino acids corresponding to CXCL12/SDF-1 beta Source: E. coli
Uniprot ID: Q9QZD1
Amino Acid Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KM
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of CXCL12/SDF-1 beta as determined by LAL method.
Predicted Molecular Mass
8.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
SDF1 beta Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 100-200 ng/ml.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-35291
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: CXCL12/SDF-1 beta
Alternate Names
SDF1 beta, SDF1b, CXCL12 beta, SDF-1 beta, SDF-1 beta/CXCL12 beta
Gene Symbol
CXCL12
Additional CXCL12/SDF-1 beta Products
Product Documents for Recombinant Rat CXCL12/SDF-1 beta Protein
Product Specific Notices for Recombinant Rat CXCL12/SDF-1 beta Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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