Recombinant Human CXCL13/BLC/BCA-1 Protein

Name: Recombinant Human CXCL13/BLC/BCA-1 Protein
Brand: Novus Biologicals
SKU: NBP2-34885

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34885

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Accession #

O43927

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL13/BLC/BCA-1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 87 amino acids corresponding to CXCL13/BLC/BCA-1 Source: E. coli

Uniprot ID: O43927

Amino Acid Sequence: VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL13/BLC/BCA-1 as determined by LAL method.

Predicted Molecular Mass

10.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL13 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-34885

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL13/BLC/BCA-1

C-X-C motif chemokine 13 encoded by the CXCL13 gene located on human chromosome 4 is constitutively expressed in secondary lymphoid organs. It is also known as B lymphocyte chemoattractant (BLC). As its name suggests, CXCL13 is selectively chemotactic for B cells belonging to both the B-1 and B-2 subsets, and elicits its effects by interacting with chemokine receptor CXCR5. It and its receptor CXCR5 control the organization of B cells within follicles of lymphoid tissues. Recombinant human CXCL13 is a single non-glycosylated polypeptide chain containing 87 amino acids and mature human BCA-1 shares 64 % amino acid sequence similarity with the murine protein and 23-34 % amino acid sequence identity with other known CXC chemokines.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13, BCA-1/CXCL13

Gene Symbol

CXCL13

UniProt