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Recombinant Mouse CXCL16 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35166

Novus Biologicals, part of Bio-Techne
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NBP2-35166-100ug
NBP2-35166-1mg
NBP2-35166-500ug
NBP2-35166-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 88 amino acids corresponding to CXCL16 Source: E. coli

Uniprot ID: Q8BSU2

Amino Acid Sequence: NQGSVAGSCS CDRTISSGTQ IPQGTLDHIR KYLKAFHRCP FFIRFQLQSK SVCGGSQDQW VRELVDCFER KECGTGHGKS FHHQKHLP

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL16 as determined by LAL method.

Predicted Molecular Mass

9.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL16 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration of 20-1000 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CXCL16 Protein

Recombinant Mouse CXCL16 Protein

SDS-Page: Recombinant Mouse CXCL16 Protein [NBP2-35166]

SDS-Page: Recombinant Mouse CXCL16 Protein [NBP2-35166]

Formulation, Preparation and Storage

NBP2-35166
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL16

CXCL16 is a member of the CXC chemokine family. Larger than other chemokines, it is one of the only two transmembrane chemokines in the family and the other is CX3CL1. Mouse CXCL16 has 246 a.a. and consists of a 26 a.a. residue putative signal peptide, an 88 a.a. residue chemokine domain, an 87 a.a. residue mucin-like spacer region, a 22 a.a. residue transmembrane domain, and a 23 a.a. residue cytoplasmic tail. Mouse CXCL16 shares 70 % sequence identity with human CXCL16 in chemokine domain. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. Functions of CXCL16 include inducing a strong chemotactic response and calcium mobilization. CXCL16 also acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis.

Alternate Names

CXCL16

Gene Symbol

CXCL16

UniProt

Additional CXCL16 Products

Product Documents for Recombinant Mouse CXCL16 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse CXCL16 Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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